![]() When exposed to light, FMN induces reactions with amino acids, polyunsaturated fatty acids, and even AH, altering the quality of nutrients. The addition of catalase suggested that lipid peroxides and H 2O 2 were produced, and the loss of thiol function suggested that an oxidation of amino acids occurred. In the lipid emulsion, peroxides increased over 3-fold in the presence of FMN or FMN + AH. However, in the presence of amino acids, FMN alone induced a generation of peroxides comparable to that observed with FMN + AH. After 24-h light exposure, dextrose solutions with admixtures of AH + FMN or AH + FMN + PS generated peroxides at concentrations similar to those observed in a 1% multivitamin solution, and over three times higher than those observed with FMN, PS, or AH alone. Thiol functions were evaluated by reduction of 5,5-dithiobis(2-nitrobenzoic acid) in the amino acid preparation. Using xylenol orange, peroxide concentrations were measured in solutions containing AH, FMN, and/or PS in water, a dextrose solution, an amino acid preparation, and a lipid emulsion. We hypothesized that the admixture of FMN or PS with electron donors generates peroxides in TPN and alters the quality of nutrients. This preparation contains two photosensitive products, 5′-phosphate flavin mononucleotide(FMN) and polysorbates (PS), as well as electron donors such as ascorbate(AH). In the presence of light, a multivitamin preparation is the main source of peroxides in solutions of total parenteral nutrition (TPN).
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